Senegal defeated Uganda 1-0 at the 2019 Africa Cup of Nations round of 16 match played on Friday night at Cairo International Stadium.
The Liverpool star became AFCON top scorer with three goals despite having played one game short and missing two penalties.
Uganda had to deal with an early blow with defender Hassan Wasswa leaving the field injured after 18 minutes.
The 27-year-old was sent clear on goal after Senegal stole the ball in Uganda territory and Mane made no mistake with a clinical low finish, that went flying beyond goalkeeper Dennis Onyango.
The Cranes would only have themselves to blame for not burying the chances in front of goal as the East African nation wasted numerous chances to equalise against the Lions of Teranga.
Mane then came close to sealing the game for Aliou Cisse after Senegal intercepted the Cranes while playing the ball out from the back and sent Mane goal bound with only Onyango to beat.
The Mamelodi Sundowns keeper brought the Liverpool star down and was lucky to escape a second booking that would have seen him sent off.
Onyango palmed Mane's spotkick denying the Premier League Golden Boot winner his brace for the night form the spot for the second time after Mane missed a penalty against Kenya.
Senegal now move one step close in their campaign to be crowned African champions and play the tournament underdogs Benin on Wednesday, July 10, at the 30 June Stadium.