The deputy minister of employment and labour relations, Baba Jamal, has lost his parliamentary seat to Ama Sey in the Akwatia Constituency.
Madam Sey, a beautician, polled 21,433 votes to beat Mr Jamal's 15,905 votes.
During campaigning, Baba Jamal had derided Madam Sey as a mere “hair dresser” who posed no threat to him.
Baba Jamal claimed that unlike him who was a professional politician and a lawyer, the New Patriotic Party's (NPP) aspirant lacked the capacity to understand proceedings in parliament and facilitate development of the constituency.
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Meanwhile Nana Akufo-Addo is leading the presidential race by almost one million votes, according to the latest provisional results.
The NPP earlier called on President Mahama to concede defeat, stressing that there was no way he could catch up with Akufo-Addo.