Captain of the Black Stars, Asamoah Gyan has been excluded from Ghana's squad to face Uganda in a World Cup qualifier on Saturday.
The Kayserispor striker was rumoured to be a doubt for the clash, having battled with injuries in recent weeks.
However, coach Kwesi Appiah still included him in his initial 26-man squad with the hope that he would recover in time to play against the Cranes.
Gyan's injury, though, appears to be more serious than first thought, and he has now been completed ruled out of the fate-defining qualifier after failing to recover.
Another notable absentee from the Ghana squad is budding winger Thomas Agyapong who also sustained a muscle tear while playing for his club.
Defenders Harrison Afful and Jonathan Mensah are also doubts for the Uganda game, as they will need to undertake late tests to prove their fitness.
Two players from the Ghana Premier League made the squad, with Vincent Atingah and Isaac Twum being rewarded with call-ups for their impressive outings in the just-ended CHAN tournament.
The Black Stars sit third in group E, behind both Uganda and Egypt.
However, they risk missing out on a World Cup spot for the first time since 2002 after wining just once in four group games.
Below is the full 24-man squad to face Uganda.
GOALKEEPERS: Richard Ofori (Martizburg United, South Africa), Lawrence Ati (Sochaux, France), Joseph Addo (Aduana Stars)
DEFENDERS: Harrison Afful (Columbus, USA), Daniel Opare (Augsburg, Germany) Agbenyenu Lumor (Portimonese, Portugal), Abass Mohammed (Harrisburg City, USA), Kasim Nuhu (Young Boys, Switzerland), Daniel Amartey (Leicester,England), Jonathan Mensah (Columbus Crew,USA), Nicholas Opoku (Club Africain, Tunisia), Vincent Atinga (Hearts of Oak)
MIDFIELDERS: Thomas Partey (Atletico Madrid, Spain), Joseph Attamah (Bakaksehir, Turkey), Christian Atsu (Newcastle United, England), Kingsley Sarfo (Malmo, Sweden), Alfred Duncan (Sassuolo FC, Italy), Ebenezer Ofori (Stuttgart, Germany), Frank Acheampong (Tianjin TEDA, China), Isaac Twum (Inter Allies), Patrick Twumasi (Astana FC, Kazakhstan)
FORWARDS: Raphael Dwamena (FC Zurich, Switzerland), John Antwi (Misr Lel-Makkasa, Egypt), Richmond Boakye Yiadom (Crvena Zvezda, Serbia)